Project title : Regulation of Ethylene - induced
نویسندگان
چکیده
Objectives: (1) Obtain complete genomic clones plus promoter regions of apple peel genes encoding HMGR isozymes HMG2 and HMG3 and determine whether expression of these genes is regulated by ethylene; (2) Demonstrate function of the gene product and obtain a genomic clone of the ethyleneresponsive promoter of the putative apple α-Farnesene Synthase gene; (3) Determine correlation among ethylene-induced transcription of genes encoding enzymes in the α-farnesene synthetic pathway, accumulation of α-farnesene during storage, and the incidence and severity of scald development; (4) Transform apple shoots with antisense constructs of HMG2, HMG3, and/or α-FS genes and test transgenic apple plants for suppression of α-farnesene production.
منابع مشابه
Project title : Regulation of Farnesene Synthesis to Control Scald PI : Bruce D . Whitaker Organization : USDA - ARS , Produce Quality and
Objectives: (1) Obtain a genomic clone of the apple α-FS gene including the promoter and ethylene response element(s); (2) Isolate and characterize the promoter region from the genomic clone of the apple peel HMG2 gene and determine whether expression of HMG2 is regulated by ethylene; (3) Evaluate the correlation of ethylene-induced transcription of genes encoding enzymes in the αfarnesene synt...
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